05-636-AF555

Anti-phospho-H2A.X Antibody (Ser139), clone JBW301, Alexa Fluor^™ 555 Conjugate

clone JBW301, from mouse, ALEXA FLUOR^™ 555

• Synonym(s): H2AXS139P, Histone H2A.X (phospho S139)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 555
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: JBW301, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): vertebrates (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NP_002096
• UniProt accession no.: P16104
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer139)
• Gene Information: human ... H2AX(3014)

Description

General description

Histone H2A.X, also known as H2A/X, or H2A.X, and encoded by the gene name H2AFX and H2AX, is a variant of histone H2A and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins. As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.

Immunogen

Linear peptide corresponding to phospho-Histone H2A.X (Ser139).

Application

Anti-phospho-H2A.X (Ser139), clone JBW301, Alexa Fluor^™ 555 Conjugate is an antibody against phospho-H2A.X (Ser139) for use in Immunocytochemistry.

Chromatin Immunoprecipitation Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 05-636) was used to detect phospho-Histone H2A.X (Ser139) by ChIP (Meier, A. et al. (2007) EMBO J., 26:2707-18).

Western Blot Analysis: 0.05-1 μg/mL of a representative lot of the non-conjugated form of this antibody (Cat. # 05-636) detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Immunocytochemistry in HeLa cells +/- Staurosporin treatment.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment.

Target description

17 kDa observed

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with 15 mg/mL BSA and 0.1% azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable