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Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA.

Food chemistry (2017-03-10)
Cui Li, Yaoyao Zhang, Sergei A Eremin, Omar Yakup, Gang Yao, Xiaoying Zhang
RÉSUMÉ

Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC50 of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC10) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.

MATÉRIAUX
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Description du produit

Sigma-Aldrich
Adjuvant complet de Freund, cell suspension
Sigma-Aldrich
Albumine from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Adjuvant incomplet de Freund, liquid
Sigma-Aldrich
Glutaraldéhyde solution, Grade II, 25% in H2O
Sigma-Aldrich
Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution