- In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.
In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.
Methods in molecular biology (Clifton, N.J.) (2022-02-12)
Pau Marfull-Oromí, Kathryn M Miller, Yimin Zou
PMID35147949
RÉSUMÉ
In vitro studies have provided valuable insights to the function and mechanisms in axon guidance. In this chapter, we will introduce the rodent "open-book" assay, pre- or postcrossing explant culture and the dissociated neuron culture. They have been used to discover mechanism which we have gone on to validate or will confirm using in vivo genetic approaches.
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Sigma-Aldrich
Gö 6976, Gö 6976, CAS 136194-77-9, is a cell-permeable, reversible, and ATP-competitive inhibitor of PKC (IC₅₀ = 7.9 nM for rat brain). Exhibits selectively for PKCα (IC₅₀ = 2.3 nM) and βI (IC₅₀ = 6.2 nM).
Sigma-Aldrich
JNK Inhibitor I, (L)-Form, Cell-Permeable, The JNK Inhibitor I, (L)-Form, Cell-Permeable controls the biological activity of JNK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.