SAB4700332

Monoclonal Anti-CD54-FITC antibody produced in mouse

clone 1H4, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-ICAM1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Conjugué: FITC conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1H4, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Technique(s): flow cytometry: suitable
• Isotype: IgG2b
• Numéro d'accès NCBI: NM_000201.2
• Numéro d'accès UniProt: P05362
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ICAM1(3383)

Description

Description générale

CD54 (cluster of differentiation 54), also known as ICAM1 (intercellular adhesion molecule 1) was initially identified as a ligand for the β2 integrin lymphocyte function-associated antigen (LFA)-1. It is a glycoprotein with a 90,000-110,000 molecular weight and shows wide range of tissue expression, including tissue macrophages and dendritic cells. It also shows expression on non-hematopoietic cells such as vascular endothelial cells, epithelial cells of thymus and mucosa and dermal fibroblasts. It is a member of the ICAM family, which also includes ICAM-2, -3, -4 and -5.

The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Immunogène

Raji cells and spleen cells fused with NS1 cells

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Actions biochimiques/physiologiques

The expression of CD54 (cluster of differentiation 54)/ICAM1 (intercellular adhesion molecule 1) is strongly increased by interleukin-1 (IL-1) and interferon (IFN)-γ. It is expressed on lymphoid cells where it is required for phorbol ester-induced aggregation. It participates in lymphocyte function-associated antigen 1 (LFA-1)-dependent NK cell stimulation through its attachment to actin cytoskeleton through α-actinin or ezrin. It also acts as the receptor for rhinovirus, a common cold virus and Plasmodium falciparum. Soluble ICAM-1 levels are elevated in pregnant women with gestational diabetes mellitus (GDM), which is an outcome of endothelial cell activation and dysfunction. Meta-analysis of case-control studies show that rs1799969 polymorphism in ICAM-1 gene is linked with increased susceptibility to cancer.

Caractéristiques et avantages

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Forme physique

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable