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Key Documents

S100HR

Sigma-Aldrich

Sephacryl®

100-HR, MW range 1000-100,000 Da (globular proteins)

Synonyme(s) :

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Numéro CAS:
65546-95-4
Numéro MDL:
MFCD00165824
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Description

stability: stable to 0.2M NaOH

Forme

suspension (20% aqueous ethanol)

Caractéristiques

autoclavable

Matrice

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

Taille des particules

25-75 μm (wet)
25-75 μm (wet)

Dimension de pores

1000-100,000 Da MW range (globular proteins)

Compatibilité

mode of use size exclusion chromatography

Température de stockage

2-8°C

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Description générale

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Application

Sephacryl® has been used:
  • to develop a novel high-throughput screening assay for sickle cell disease drug discovery
  • to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Informations légales

Sephacryl is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

100.4 - 109.4 °F - closed cup

Point d'éclair (°C)

38 - 43 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Marc A Benson et al.
Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
Diederik Johannes Opperman et al.
Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt
Matthew Lord et al.
The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
D R Shackleton et al.
The Biochemical journal, 266(3), 917-919 (1990-03-15)
A method is described for the purification of piglet skin lysyl oxidase from a crude urea extract by two simple chromatographic procedures. The method relies on the selective interaction between Sephacryl S-200 and lysyl oxidase that occurs in the absence
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