R6028

Anti-ROCK-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-Rho-kinase
• Numéro MDL: MFCD02264068
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 160 kDa
• Espèces réactives: human, rat, mouse
• Technique(s): immunoprecipitation (IP): 10-20 μL using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line; microarray: suitable; western blot: 1:1,000-1:2,000 using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
• Numéro d'accès UniProt: Q13464
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ROCK1(6093) ; mouse ... Rock1(19877) ; rat ... Rock1(81762)

Description

Description générale

Rho-associated coiled-coil protein kinase 1 or ROCK-1 is a serine/threonine kinase that regulates actin assembly, cell movement and contraction. This kinase activates the JNK signaling pathway, modulates stress granule formation and regulates apoptosis in cardiac myocytes and stress granules .

Immunogène

synthetic peptide corresponding to amino acids 1336-1354 located at the C-terminus of human ROCK-1, conjugated to KLH. This sequence is identical in mouse and rat ROCK-1.

Application

Anti-ROCK-1 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Actions biochimiques/physiologiques

Rho-associated kinases (ROCK or Rho-kinase) is implicated in Rho-mediated actin reorganization such as formation of stress fibres and focal adhesions and smooth muscle contraction. Rho-kinases lead to an increase of myosin light chain (MLC) phosphorylation and thereby induce actomyosin based contractility. ROCK accumulates at the cleavage furrow during cytokinesis. In addition, a dominant negative form of ROCK increases multinuclei in mammalian cells. ROCK directly phosphorylates and activates LIM-kinase (LIMK), resulting in downstream phosphorylation and inaction of the actin depolymerizing factor or cofilin.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable