P3415

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

≥95%, powder

• Synonyme(s) : N-Succinimidyl 3-(2-pyridyldithio)propionate, SPDP
• Formule empirique (notation de Hill): C₁₂H₁₂N₂O₄S₂
• Numéro CAS: 68181-17-9
• Poids moléculaire : 312.36
• Numéro Beilstein : 1548137
• Numéro CE : 269-034-3
• Numéro MDL: MFCD00009636
• Code UNSPSC : 12352106
• ID de substance PubChem : 24898409
• Nomenclature NACRES : NC.07

Propriétés

• Pureté: ≥95%
• Forme: powder
• Pertinence de la réaction: reagent type: cross-linking reagent
• Pf: 84-86 °C (lit.)
• Solubilité: acetone: 50 mg/mL; DMF: soluble
• Groupe fonctionnel: NHS ester
• Température de stockage: −20°C
• Chaîne SMILES: O=C(CCSSC1=NC=CC=C1)ON2C(CCC2=O)=O
• InChI: 1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
• Clé InChI: JWDFQMWEFLOOED-UHFFFAOYSA-N

Description

Description générale

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker.

Application

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
It has been used for antibody conjugation and as a protein cross-linker.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves