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P3369

Sigma-Aldrich

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

Synonyme(s) :

Salmine sulfate from salmon sperm, Protamine sulfate

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About This Item

Numéro MDL:
MFCD00132091
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Protamine sulfate, meets USP testing specifications

Agence

meets USP testing specifications

Pureté

≥92% (chromatography)
90.0-110.0% dry basis

Forme

solid

Poids mol.

Mr 5000-10000

Technique(s)

transduction: suitable

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Application

Protamine sulfate has been used in the transduction of
  • lentiviruses into human embryonic 293T cells
  • shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
  • lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells

Actions biochimiques/physiologiques

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Moosung Lee et al.
eLife, 9 (2020-12-18)
The immunological synapse (IS) is a cell-cell junction between a T cell and a professional antigen-presenting cell. Since the IS formation is a critical step for the initiation of an antigen-specific immune response, various live-cell imaging techniques, most of which
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
V Diness et al.
Thrombosis and haemostasis, 56(3), 318-322 (1986-12-15)
The neutralization of a low molecular weight heparin (LHN-1) and conventional heparin (CH) by protamine sulfate has been studied in vitro and in vivo. In vitro, the APTT activity of CH was completely neutralized in parallel with the anti-Xa activity.
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
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