MAK491

Formate Assay Kit

sufficient for 100 colorimetric tests

• Synonyme(s) : Formate Test Kit
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Application(s): environmental
• Méthode de détection: colorimetric
• Maladie(s) pertinente(s): neurological disorders
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Formate is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in the body, and the eventual metabolic byproduct of methanol, which is first broken down to formaldehyde. At high levels, formate is neurotoxic to the central nervous system and can cause blindness, coma, and death. Although naturally present in the body at low levels, elevated levels of formate may be used as an indicator of formaldehyde exposure and methanol poisoning.

Caractéristiques et avantages

• Linear detection range 0.050 to 5 mM formate.
• Compatible with high-throughput handling systems.

Adéquation

The kit is suitable for the determination of formate in biological samples such as urine and serum.

Principe

The Formate Assay Kit is based on the formate dehydrogenase catalyzed oxidation of formate, which generates carbon dioxide and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to the formate concentration in the sample.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2