Accéder au contenu
Merck
Toutes les photos(1)

Documents

MAK329

Sigma-Aldrich

L-Lactate Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

L-Lactic Acid Assay Kit, Lactate Quantification Kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Entrée

cell
plasma
serum
solution(s) (collagen)

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; cancer

Température de stockage

−20°C

Description générale

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Application

The L-Lactate Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Endocrinological Disease Research

Caractéristiques et avantages

Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adéquation

Suitable for lactate determination in serum, plasma, and cell media samples.

Principe

The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Code de la classe de stockage

10 - Combustible liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Katharina Richard et al.
The Journal of experimental medicine, 218(2) (2020-11-21)
Two cosegregating single-nucleotide polymorphisms (SNPs) in human TLR4, an A896G transition at SNP rs4986790 (D299G) and a C1196T transition at SNP rs4986791 (T399I), have been associated with LPS hyporesponsiveness and differential susceptibility to many infectious or inflammatory diseases. However, many
Triiodothyronine prevents tissue hypoxia in experimental sepsis: potential therapeutic implications.
Iordanis S Mourouzis et al.
Intensive care medicine experimental, 9(1), 17-17 (2021-04-10)
Ana Zúñiga et al.
ACS synthetic biology, 10(12), 3527-3536 (2021-12-02)
Bacteria equipped with genetically encoded lactate biosensors are promising tools for biopharmaceutical production, diagnostics, and cellular therapies. However, many applications involve glucose-rich and anoxic environments, in which current whole-cell lactate biosensors show low performance. Here we engineer an optimized, synthetic
Tia R Tidwell et al.
Cancer & metabolism, 10(1), 9-9 (2022-05-17)
Most in vitro cancer cell experiments have been performed using 2D models. However, 3D spheroid cultures are increasingly favored for being more representative of in vivo tumor conditions. To overcome the translational challenges with 2D cell cultures, 3D systems better

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique