MAK083
Glucose Uptake Colorimetric Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
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Utilisation
sufficient for 100 colorimetric tests
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; cancer
Température de stockage
−20°C
Description générale
Glucose is the primary source of energy for most cells. Glucose uptake into cells is highly regulated and the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).
Application
Glucose uptake colorimetric assay kit has been used to measure glucose uptake by a variety of cells.
Adéquation
Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.
Principe
The Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which is then determined by a recycling amplification reaction in which the NADPH is utilized by glutathione reductase in a coupled enzymatic reaction that produces glutathione. Glutathione reacts with DTNB to product TNB, which is detected at 412 nm.
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Kyung-Ah Cho et al.
International journal of molecular medicine, 36(3), 839-844 (2015-07-15)
Impaired lipid metabolism and inflammatory pathways have individually been implicated in the development of insulin resistance in skeletal muscle; however, little evidence is available to date linking the two in this context. In this study, we explored a potential molecular
Huijuan Shi et al.
Frontiers in oncology, 10, 1034-1034 (2020-08-09)
Colon cancer is one of the most prevalent malignancies that lead to high occurrence of cancer-related deaths. Currently, chemotherapies and radiotherapies remain the primary treatments for advanced colon cancer. Despite the initial effectiveness, a fraction of colon cancer patients developed
Behrouz Etesami et al.
Reports of biochemistry & molecular biology, 9(1), 14-25 (2020-08-22)
Obesity, often associated with insulin resistance and type 2 diabetes, is a metabolic disease that can result in dyslipidemia and hyperglycemia. Many reports describe the hypoglycemic and hypolipidemic properties of the Phoenix dactylifera L. seed extract in STZ-induced diabetic rat
PLIN2 inhibits insulin-induced glucose uptake in myoblasts through the activation of the NLRP3 inflammasome.
Cho K A and Peter B K
International Journal of Molecular Medicine, 36(3), 839-844 (2015)
MicroRNA-138 suppresses proliferation, invasion and glycolysis in malignant melanoma cells by targeting HIF-1α.
Chen Y, et al.
Experimental and Therapeutic Medicine, 11(6), 2513-2518 (2016)
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