M9400
β-Mannosidase from Helix pomatia
5-30 units/mL, ammonium sulfate suspension, crude extract
Synonyme(s) :
β-D-Mannoside mannohydrolase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32
Produits recommandés
Forme
ammonium sulfate suspension
Qualité
crude extract
Poids mol.
94 kDa
Concentration
5-30 units/mL
Activité étrangère
β-N-acetylglucosaminidase and α-mannosidase <1.0%
Température de stockage
2-8°C
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Description générale
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
Application
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
Actions biochimiques/physiologiques
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
Définition de l'unité
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Forme physique
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
Inhibiteur
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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