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Key Documents

L5520

Sigma-Aldrich

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

Leibovitz L15

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Reconstitution

Supplement with 0.3 g/L L-glutamine.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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The Journal of cell biology, 156(2), 349-359 (2002-01-25)
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B Houchmandzadeh et al.
The Journal of cell biology, 139(1), 1-12 (1997-10-06)
The structure of mitotic chromosomes in cultured newt lung cells was investigated by a quantitative study of their deformability, using micropipettes. Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times

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