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H5912

Sigma-Aldrich

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p

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About This Item

Numéro MDL:
MFCD04974783
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 15 kDa

Espèces réactives

mouse, human

Technique(s)

microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine

Numéro d'accès UniProt

P16104

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pSer139)

Informations sur le gène

human ... H2AFX(3014)
mouse ... H2afx(15270)

Description générale

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

Immunogène

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.
synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139).

Application

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

Actions biochimiques/physiologiques

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

ATM-dependent DNA damage-independent mitotic phosphorylation of H2AX in normally growing mammalian cells
McManus KJ and Hendzel MJ
Molecular Biology of the Cell, 16(10), 5013-5025 (2005)
Resveratrol decreases Rad51 expression and sensitizes cisplatin-resistant MCF-7 breast cancer cells
Leon-Galicia I, et al.
Oncology Reports, 39(6), 3025-3033 (2018)
Kalyan Mahapatra et al.
Scientific reports, 11(1), 11659-11659 (2021-06-04)
As like in mammalian system, the DNA damage responsive cell cycle checkpoint functions play crucial role for maintenance of genome stability in plants through repairing of damages in DNA and induction of programmed cell death or endoreduplication by extensive regulation
Genomic instability in mice lacking histone H2AX
Celeste A, et al.
Science (New York, N.Y.), 296(5569), 922-927 (2002)
H2AX: functional roles and potential applications
Dickey J, et al.
Chromosoma, 118(6), 683-692 (2009)
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