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Merck
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Key Documents

G6784

Sigma-Aldrich

ʟ-Glutamine–Penicillin–Streptomycin

solution, suitable for cell culture, BioReagent

Synonyme(s) :

Pen/Strep, Penicillin/Streptomycin 

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About This Item

Numéro MDL:
MFCD00283636
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.76

product name

L-Glutamine–Penicillin–Streptomycin solution, L-glutamine 200 mM, streptomycin 10 mg/mL, penicillin 10,000 units, 0.1 μm filtered, BioReagent, suitable for cell culture

Source biologique

synthetic

Niveau de qualité

300

Description

stabilized

Stérilité

0.1 μm filtered

Gamme de produits

BioReagent

Forme

liquid

Composition

L-glutamine, 200 mM
penicillin, 10,000 units
streptomycin, 10 mg/mL

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria

Mode d’action

cell wall synthesis | interferes
protein synthesis | interferes

Température de stockage

−20°C

Catégories apparentées

Application

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Actions biochimiques/physiologiques

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Composants

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Zhengliang L Wu et al.
Glycobiology, 30(7), 454-462 (2020-01-04)
Cells are covered with glycans. The expression and distribution of specific glycans on the surface of a cell are important for various cellular functions. Imaging these glycans is essential to aid elucidation of their biological roles. Here, utilizing methods of
N Massa et al.
Canadian journal of microbiology, 64(10), 647-663 (2018-05-11)
Candida glabrata is an opportunistic pathogen, associated with endocarditis, meningitis, and disseminated disease, and also with complicated vaginitis. Essential oils derived from aromatic plants are known in traditional medicine as antimicrobial agents and have antifungal properties. The aim of this
Nikoletta Sztán et al.
Reproduction, fertility, and development, 29(11), 2206-2216 (2017-03-28)
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we
Miriam Gonzalez Gonzalez et al.
Journal of immunological methods, 457, 73-77 (2018-02-25)
Standard cell culturing on plastic plates (two dimensional (2D) cultures) does not represent the actual microenvironment where cells reside in tissues. The three dimensional (3D) systems, composed of extracellular matrix and/or pure amino acids which form a scaffold for cells
Kate M Herum et al.
Journal of the American Heart Association, 9(3), e013518-e013518 (2020-02-01)
Background Pressure overload of the heart occurs in patients with hypertension or valvular stenosis and induces cardiac fibrosis because of excessive production of extracellular matrix by activated cardiac fibroblasts. This initially provides essential mechanical support to the heart, but eventually
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