Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

G3907

Millipore

Agarose−glutathion

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Synonyme(s) :

Agarose glutathion à liaison S, Agarose-GSH

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00131200
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Forme

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Classe(s) chimique(s) de l'analyte

proteins (GST)

Conditionnement

set of 3 pre-packed columns (2.5 ml each)

Technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

Matrice

cross-linked 4% beaded agarose

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer.

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
La chromatographie d'affinité avec de l′agarose-glutathion assure une purification rapide, non dénaturante, en conditions douces et hautement sélective des protéines contenant des séquences de liaison au glutathion, telles que la glutathion S-transférase (GST), la glutathion peroxydase et la glyoxalase I.

Liaison

Suspension of Product No. G 4510

Forme physique

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

180.1 °F

Point d'éclair (°C)

82.3 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 4

1 of 4

GST Bulk Kit Cytiva 27-4570-01, pack of 1 kit

GE27-4570-01

GST Bulk Kit

Glutathione Sepharose™ High Performance Cytiva 17-5279-01, pack of 25 mL

GE17-5279-01

Glutathione Sepharose™ High Performance

Protéase PreScission Cytiva 27-0843-01

GE27-0843-01

Protéase PreScission

L-Glutathion réduit ≥98.0%

Sigma-Aldrich

G4251

L-Glutathion réduit

F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique