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FP0010

Sigma-Aldrich

FluoroProfile Protein Quantification Kit

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About This Item

Code UNSPSC :
12352200

Conditions d'expédition

wet ice

Température de stockage

−20°C

Application

Reversible covalent binding to proteins by the fluorophore epicocconone provides a powerful and sensitive method for protein quantification

Caractéristiques et avantages

  • Yields an intense fluorescent red on protein binding
  • Binding is completely reversible making the protein suitable for mass spectrometry and functional assays
  • Water soluble proteins may be quantified without using organic solvents
  • A large dynamic range, large Stokes shift and highly sensitive
  • Highly tolerant of substances commonly interfering with protein quantification
  • Biodegradable and environmentally friendly

Autres remarques

For the quantification of total protein in solution by the interaction of a unique fluorophore with the amines of polypeptides.

Informations légales

FluoroProfile is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • P5619Protein Standard, analytical standard, 2 mg/vial BSAFDS

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ondřej Kaplan et al.
PloS one, 11(10), e0163697-e0163697 (2016-10-08)
We describe the production of a highly-active mutant VEGF variant, α2-PI1-8-VEGF121, which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α2-PI1-8-VEGF121 gene was synthesized, cloned into a pET-32a(+) vector and
R P Ubrihien et al.
Archives of environmental contamination and toxicology, 82(4), 520-538 (2022-04-21)
The effects of multigenerational Cu exposure on the freshwater gastropod Isidorella newcombi were investigated. Snails were exposed to a range of treatment-specific Cu concentrations in the parental to F2 generations, and a common Cu concentration in the F3 generation. In
Phillip J L Bell et al.
Journal of the American Chemical Society, 125(31), 9304-9305 (2003-08-02)
Epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus Epicoccum nigrum. Epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long Stokes' shift that will
Jiaorong Tan et al.
Journal of diabetes research, 2019, 5483946-5483946 (2019-06-22)
This study is aimed at evaluating the effects, functions, and mechanism of HNF1α on hepatic glycolipid metabolism. In this study, free fatty acid- (FFA-) induced steatosis of hepatocyte liver cell LO2 was used as an in vitro model. The methods
Helena A Schmitz et al.
Aquatic toxicology (Amsterdam, Netherlands), 160, 22-30 (2015-01-13)
Metals are accumulated by filter feeding organisms via water, ingestion of suspended sediments or food. The uptake pathway can affect metal toxicity. Saccostrea glomerata were exposed to cadmium through cadmium-spiked suspended sediments (19 and 93μg/g dry mass) and cadmium-enriched phytoplankton

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