FG0100
Kits et réactifs pour triglycérides et glycérol à l'état libre
1 kit sufficient for 1000 reactions
Synonyme(s) :
Triglyceride and Free Glycerol Kits and Reagents
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26
Produits recommandés
Utilisation
kit sufficient for 1000 reactions
Méthode de détection
colorimetric
Température de stockage
2-8°C
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Description générale
Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
Application
Les kits pour triglycérides et glycérol à l′état libre sont utilisés pour la détermination quantitative du glycérol, des triglycérides totaux et des triglycérides à l′état libre.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.
Adéquation
Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
Liaison
En plus des kits, les réactifs individuels et le standard de glycérol sont disponibles pour réaliser un plus petit nombre de réactions.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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