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CS0001

Sigma-Aldrich

Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)

sufficient for 200 colorimetric or fluorometric tests

Synonyme(s) :

DDPC, DEPC, DLOPC, DLPC, DMPC, DOPC, DPPC, DSPC, EPC, HEPC, HSPC, LYSOPC, MPPC, MSPC, PC, PMPC, POPC, PSPC, Phosphatidylcholine, SMPC, SOPC, lecithin, lysolecithin, sphingomyelin

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 200 colorimetric or fluorometric tests

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline.

The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).

The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).

Application

  • Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
  • Enables evaluation of drug effects on phospholipids metabolism

Caractéristiques et avantages

Our economical Phospholipid quantification assay kit also saves your time and efforts:

  • Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples
  • User friendly: No need to weigh or calculate needed amounts of reagents on your own
  • Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 200 tests.
  • Suitable for both colorimetric and fluorometric detection
  • Detection time: only 30 minutes

Adéquation

Suitable for the detection of phospholipids in biological samples

Pictogrammes

FlameHealth hazard
GHS02,GHS08

Mention d'avertissement

Danger

Mentions de danger

H225,H317,H319,H334,H412

Classification des risques

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Sophie Lau et al.
Angewandte Chemie (International ed. in English), 59(41), 18126-18130 (2020-06-17)
The biological function of high-density lipoprotein (HDL) nanoparticles, the so-called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol
Sophie Lau et al.
Physical chemistry chemical physics : PCCP, 24(38), 23651-23660 (2022-09-23)
Cholesterol is an essential component of eukaryotic cellular membranes that regulates the order and phase behaviour of dynamic lipid bilayers. Although cholesterol performs many vital physiological roles, hypercholesterolaemia and the accumulation of cholesterol in atherosclerotic plaques can increase the risk
Tejaswini Appidi et al.
Diagnostics (Basel, Switzerland), 13(8) (2023-05-16)
This paper reports the colorimetric analysis of cervical-cancer-affected clinical samples by the in situ formation of gold nanoparticles (AuNPs) formed with cervico-vaginal fluids collected from healthy and cancer-affected patients in a clinical setup, termed "C-ColAur". We evaluated the efficacy of
Mehdi Zouiouich et al.
The Journal of cell biology, 221(6) (2022-04-08)
Membrane contact sites between organelles are organized by protein bridges. Among the components of these contacts, the VAP family comprises ER-anchored proteins, such as MOSPD2, that function as major ER-organelle tethers. MOSPD2 distinguishes itself from the other members of the
Fei Luo et al.
Cellular and molecular gastroenterology and hepatology, 13(3), 879-899 (2021-12-20)
Substitution of lysine for glutamic acid at residu 167 in Transmembrane 6 superfamily member 2 (TM6SF2) is associated with fatty liver disease and reduced plasma lipid levels. Tm6sf2-/- mice replicate the human phenotype but were not suitable for detailed mechanistic

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