M3 Clone M-3

93120827, mouse skin, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: M3 Clone M-3, 93120827
• Source biologique: mouse skin
• Mode de croissance: Adherent
• Caryotype: Modal no. 83; hypertetraploid
• Morphologie: Epithelial
• Produits: Melanin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Cloudman S 91 melanoma

Description de la lignée cellulaire

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Application

Tumourigenicity studies

Milieu de culture

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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