EPC

93120820, fish skin, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: EPC, from fish skin, 93120820
• Source biologique: fish skin
• Mode de croissance: Adherent
• Caryotype: 2n = 96
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Fish epithelioma Fathead minnow Pimephales promelas

Description de la lignée cellulaire

This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.

Profil d'ADN

Not specified

Milieu de culture

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Autres remarques

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