NCTC clone 1469

88111403, mouse liver, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: NCTC clone 1469, 88111403
• Source biologique: mouse liver
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C3H/An liver

Description de la lignée cellulaire

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Application

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Milieu de culture

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

Autres remarques

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