CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: CRFK (BVD Ag negative), 86093002
• Source biologique: feline kidney
• Mode de croissance: Adherent
• Caryotype: Hypodiploid, modal no 36
• Morphologie: Epithelial
• Produits: Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Cat kidney

Description de la lignée cellulaire

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Application

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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