C9281
Cholesterol Esterase from Pseudomonas fluorescens
lyophilized powder, ≥10,000 units/g protein
Synonyme(s) :
CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
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About This Item
Produits recommandés
Source biologique
Pseudomonas fluorescens
Niveau de qualité
Pureté
10-30% (TCA-Biuret)
Forme
lyophilized powder
Activité spécifique
≥10,000 units/g protein
Poids mol.
~129 kDa
Composition
Protein, ~20%
Conditions de stockage
under inert gas (argon)
Technique(s)
cell based assay: suitable
Couleur
tan to brown
pH
7-9
Solubilité
0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
Température de stockage
−20°C
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Description générale
Research area: Cell Signaling
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Application
Cholesterol esterase from Pseudomonas fluorescens has been used in:
- in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
- a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
- an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
- for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
- to hydrolyze native cholesterol ester (CE) during filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.
Actions biochimiques/physiologiques
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..
Autres remarques
Contains potassium phosphate and TRITON® X-100.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.
Remarque sur l'analyse
Protein determined by biuret.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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