B5264

Kanamycin B sulfate salt

aminoglycoside antibiotic

• Synonyme(s) : Bekanamycin sulfate salt
• Formule empirique (notation de Hill): C₁₈H₃₇N₅O₁₀ · xH₂SO₄
• Poids moléculaire : 483.51 (free base basis)
• Numéro Beilstein : 5235274
• Numéro MDL: MFCD00078511
• Code UNSPSC : 51281654
• ID de substance PubChem : 24891829
• Nomenclature NACRES : NA.85

Propriétés

• Forme: powder or crystals
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria; mycoplasma
• Mode d’action: protein synthesis | interferes
• Température de stockage: −20°C
• Chaîne SMILES: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](N)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H37N5O10.H2O4S/c19-2-6-11(26)12(27)9(23)17(30-6)32-15-4(20)1-5(21)16(14(15)29)33-18-13(28)8(22)10(25)7(3-24)31-18;1-5(2,3)4/h4-18,24-29H,1-3,19-23H2;(H2,1,2,3,4)/t4-,5+,6+,7+,8-,9+,10+,11+,12+,13+,14-,15+,16-,17+,18+;/m0./s1
• Clé InChI: YGTPKDKJVZOVCO-KELBJJLKSA-N

Description

Description générale

Chemical structure: aminoglycoside

Application

Used as a selection agent for cells transformed with kanamycin B (neoR, kanR) resistance gene.

Actions biochimiques/physiologiques

Kanamycin B is an aminoglycoside antibiotic that is the 2-amino,2-deoxy-analog of kanamycin A. Mode of Action: Binds to 70S ribosomal subunit; inhibits translocation; elicits miscoding. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasm.

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial Spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Notes préparatoires

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)