A5763

Adenosine 3′,5′-diphosphate disodium salt

≥96%

• Synonyme(s) : 3′-phosphoadenosine 5′-phosphate, 3′-phosphorylated nucleotide, PAP, 3′-Phosphoadenosine 5′-phosphate
• Formule empirique (notation de Hill): C₁₀H₁₃N₅Na₂O₁₀P₂
• Numéro CAS: 75431-54-8
• Poids moléculaire : 471.16
• Numéro MDL: MFCD00057007
• Code UNSPSC : 41106305
• eCl@ss : 32160414
• ID de substance PubChem : 24891020
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: synthetic (inorganic)
• Niveau de qualité: 200
• Pureté: ≥96%
• Forme: powder
• Solubilité: water: 25 mg/mL, clear, colorless to very faintly yellow
• Température de stockage: −20°C
• Chaîne SMILES: [Na].Nc1ncnc2n(cnc12)C3OC(COP(O)(O)=O)C(OP(O)(O)=O)C3O
• InChI: 1S/C10H15N5O10P2.Na.H/c11-8-5-9(13-2-12-8)15(3-14-5)10-6(16)7(25-27(20,21)22)4(24-10)1-23-26(17,18)19;;/h2-4,6-7,10,16H,1H2,(H2,11,12,13)(H2,17,18,19)(H2,20,21,22);;
• Clé InChI: ISROZYFZEAVMSP-UHFFFAOYSA-N

Description

Description générale

3′-phosphoadenosine 5′-phosphate (PAP), a 3′-phosphorylated nucleotide is found in almost all organisms and is obtained as a by-product of sulfur and lipid metabolism.

Application

Adenosine 3′,5′-diphosphate disodium salt has been used:

• to spot sample on cellulose high-performance thin-layer chromatography (HPTLC) plates in two-dimensional thin layer chromatography
• in enzyme activity assay to study the activities of HOS2/FIERY1 wild type
• hos2 mutant and fiery1?2 mutant protein against 3′-phosphoadenosine 5′-phosphate (PAP)
• as a standard for the quantification of phosphoadenosines

Actions biochimiques/physiologiques

3′-phosphoadenosine 5′-phosphate (PAP) is capable of blocking exoribonucleases (XRNs) activity in the nucleus and cytosol. It stimulates stomatal closure and can serve as a secondary messenger during abscisic acid (ABA) signaling. It is capable of blocking RNA catabolism. Hence it may serve as a physiological modulator of poly (ADP-ribose) polymerase 1 (PARP1) activity.

Adenosine 3′,5′-diphosphate (PAP) is used to study the kinetics and mechanisms of hydroxysteroid sulfotransferases such as SULT1A1, SULT2A1 of which it is product inhibitor.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves