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32884

Sigma-Aldrich

Giemsa Stain, Modified Solution

according to Giemsa

Synonyme(s) :

Azure eosin methylene blue, Giemsa solution

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About This Item

Numéro CAS:
51811-82-6
Numéro MDL:
MFCD00081642
Code UNSPSC :
12171500
ID de substance PubChem :
329754377
Nomenclature NACRES :
MA.02

Forme

liquid

Niveau de qualité

200

Qualité

according to Giemsa

Technique(s)

UV/Vis spectroscopy: suitable

Couleur

dark blue

pH

6.9

Densité

1.036 g/cm3 at 20 °C

Adéquation

passes test for UV spectrum

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

Clé InChI

NALREUIWICQLPS-UHFFFAOYSA-N

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Description générale

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Autres remarques

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principe

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Pictogrammes

FlameSkull and crossbonesHealth hazard
GHS02,GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Organes cibles

Eyes,Central nervous system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

51.8 °F - closed cup

Point d'éclair (°C)

11 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Xiao Liu et al.
EBioMedicine, 46, 42-53 (2019-08-10)
Twist-related protein 1 (TWIST1) plays an essential role in the carcinogenesis and metastasis of NSCLC. Our aims were to identify the molecule at the downstream of TWIST1 and to evaluate its potential as a diagnostic and a prognostic marker in
S R Patil et al.
Science (New York, N.Y.), 173(3999), 821-822 (1971-08-27)
Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the
Yonglian Zeng et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 125, 110029-110029 (2020-02-29)
Faciogenital Dysplasia 1 (FGD1) has been involved in a variety of biological processes, including cytoskeleton restructuring, cell morphology, cell cycle progression, and cell polarity. Abnormal expression of FGD1 was also identified in several types of cancers, indicating its critical role
Therese Wilhelm et al.
Nature communications, 10(1), 3585-3585 (2019-08-10)
Replication stress, a hallmark of cancerous and pre-cancerous lesions, is linked to structural chromosomal aberrations. Recent studies demonstrated that it could also lead to numerical chromosomal instability (CIN). The mechanism, however, remains elusive. Here, we show that inducing replication stress
Zhenya Guo et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 124, 109857-109857 (2020-01-24)
TRAF-interacting protein (TRAIP) is a RING-type E3 ubiquitin ligase which has been implicated in various cellular processes, including NF-κB activation, DNA damage response, mitosis, and tumorigenesis. It is considered as a tumor suppressor in basal cell carcinomas and breast cancer
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