70191
Mueller Hinton Agar
suitable for microbiology, NutriSelect® Plus
Synonyme(s) :
MH agar; Antibiotic sensitivity test media; disc diffusion test media
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About This Item
Code UNSPSC :
41171607
Nomenclature NACRES :
NA.74
Produits recommandés
Stérilité
non-sterile
Niveau de qualité
Gamme de produits
BioChemika
Forme
powder
Durée de conservation
limited shelf life, expiry date on the label
Composition
agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L
Fabricant/nom de marque
NutriSelect® Plus
Technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
pH final
7.3±0.2 (25 °C)
Application(s)
clinical testing
food and beverages
microbiology
Température de stockage
10-30°C
Adéquation
nonselective for Neisseria spp.
nonselective for bacteria (General Media)
Description générale
Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:
- It shows good reproducibility from batch to batch
- It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
- It supports the growth of both fastidious and non-fastidious bacterial pathogens
- The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
- It is a loose agar and therefore facilitates better diffusion of antibiotics
- In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
- In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
- In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli
Application
Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.
Notes préparatoires
Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.
Autres remarques
Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar
Note de bas de page
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Informations légales
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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