11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Synonyme(s) :
RNA labeling
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About This Item
Code UNSPSC :
41105500
Produits recommandés
Forme
solution
Niveau de qualité
Utilisation
sufficient for 20 reactions
Conditionnement
pkg of 40 μL
Fabricant/nom de marque
Roche
Technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
Température de stockage
−20°C
Description générale
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Spécificité
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Application
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Qualité
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principe
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Notes préparatoires
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
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