MABE329

Anti-c-Fos Antibody, clone 2G9C3

clone 2G9C3, from mouse

• Synonyme(s) : Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2G9C3, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_005243
• Numéro d'accès UniProt: P01100
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FOS(2353)

Description

Description générale

c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Spécificité

This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins.

Immunogène

Linear peptide corresponding to human c-Fos.

Application

Anti-c-Fos Antibody, clone 2G9C3 is a Mouse Monoclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB, ICC.

Immunocytochemistry Analysis: A representative lot detected c-Fos in schwann cells. (Lafarga, M., et al. (2002). Molecular Biology of the Cell. 13:2771–2782.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.

Description de la cible

~55/62 kDa observed. This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. The calculated molecular wieght is 46 kDa; however, c-Fos has been observed at ~55 kDa in western blots. (Assim, M., et al. (2010). Journal of Biological Chemistry. 285(26):20343–20357.)

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ from cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable