MABE1020

Anti-Acetyl Lysine, Pan, clone RM101 Antibody

clone RM101, from rabbit, purified by affinity chromatography

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: RM101, monoclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): all (based on 100% sequence homology)
• Technique(s): dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

DNA is tightly packed in the nucleus into nucleosomes, generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Immunogène

Acetyl lysine-BSA corresponding to all species.

Application

Anti-Acetyl Lysine, Pan, clone RM101 is an antibody against Acetyl Lysine, Pan for use in WB, Dot Blot, ICC, IP.

Immunocytochemistry Analysis: A representative lot detected Acetyl Lysine, Pan in A431 cells.
Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected Acetyl Lysine, Pan in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Acetyl Lysine, Pan in A431 cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Qualité

Evaluated by Western Blotting in Trichostatin A (TSA) treated HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 of this antibody detected Acetyl Lysine, Pan in Trichostatin A (TSA) treated HeLa cell lysate.

Forme physique

Protein A purified

Purified rabbit monoclonal IgG in buffer containing PBS with 1% BSA and 0.09% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable