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Key Documents

MAB4360A4

Sigma-Aldrich

Anti-TRA-1-60 Antibody, clone TRA-1-60, Alexa Fluor 488 Conjugate

clone TRA - 1-60, from mouse, ALEXA FLUOR 488

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

ALEXA FLUOR 488

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

TRA - 1-60, monoclonal

Espèces réactives

human

Technique(s)

immunocytochemistry: suitable

Isotype

IgM

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PODXL(5420)

Description générale

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Spécificité

This antibody reacts with TRA-1-60 antigen expressed upon the surface of human EC, EG, and ES cells. No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-81 (MAB4381A4) and TRA-1-60 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan.

Immunogène

Human embryonal carcinoma cell line 2102Ep

Application

Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, Alexa Fluor 488 Conjugate validated for use in IC.
Research Category
Neuroscience

Qualité

Evaluated by Immunocytochemistry in H9 human embryonic stem cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.

Forme physique

Purified mouse monoclonal IgM conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
size exclusion

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
H9 human embryonic stem cells

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Yohei Hayashi et al.
Communications biology, 1, 218-218 (2018-12-12)
Conventional cell handling and sorting methods require manual labor, which decreases both cell quality and quantity. To purify adherent cultured cells, cell purification technologies that are high throughput without dissociation and can be utilized in an on-demand manner are expected.
Kung-Kai Kuo et al.
Stem cells (Dayton, Ohio), 34(11), 2613-2624 (2016-06-25)
The network of stemness genes and oncogenes in human patient-specific reprogrammed cancer stem cells (CSCs) remains elusive, especially in liver cancer. HepG2-derived induced pluripotent stem cell-like cells (HepG2-iPS-like cells) were generated by introducing Yamanaka factors and the knockdown vector shTP53.
Ning Sun et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15720-15725 (2009-10-07)
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here
Vanessa Sauer et al.
Cell transplantation, 25(12), 2221-2243 (2016-08-12)
Although several types of somatic cells have been reprogrammed into induced pluripotent stem cells (iPSCs) and then differentiated to hepatocyte-like cells (iHeps), the method for generating such cells from renal tubular epithelial cells shed in human urine and transplanting them
Zhongwen Li et al.
Cell death & disease, 10(10), 763-763 (2019-10-12)
Hepatocytes have been successfully generated from human pluripotent stem cells (hPSCs). However, the cost-effective and clinical-grade generation of hepatocytes from hPSCs still need to be improved. In this study, we reported the production of functional hepatocytes from clinical-grade human embryonic

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