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ESG1107

Sigma-Aldrich

Protéine LIF de souris recombinante ESGRO®

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.

Synonyme(s) :

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160801
Nomenclature NACRES :
NA.75

Niveau de qualité

100

Pureté

>95% (active component mLIF, SDS-PAGE)

Forme

liquid

Fabricant/nom de marque

Chemicon®

Concentration

10000000 units/mL

Technique(s)

cell culture | stem cell: suitable

Entrée

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

Numéro d'accès NCBI

NM_008501

Numéro d'accès UniProt

P09056

Conditions d'expédition

wet ice

Informations sur le gène

mouse ... LIF(16878)

Catégories apparentées

Description générale

Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Application



SUGGESTED PROTOCOLS:

ES Cells: Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

Forme physique

Format : purifié

Notes préparatoires

®

Stockage et stabilité

A cold pack may be included for customer relations, however it is truly not needed. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. ° Freeze-thawing will reduce potency. ° Freeze-thawing should be avoided. °

Remarque sur l'analyse

Activité spécifique: ®& μ

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay:

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Xudong Fu et al.
Science advances, 6(22), eaay5181-eaay5181 (2020-06-12)
In mouse embryonic stem cell (ESC), a small cell population displays totipotent features by expressing a set of genes that are transiently active in 2-cell-stage embryos. These 2-cell-like (2C-like) cells spontaneously transit back into the pluripotent state. We previously dissected
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Janebodin, Kajohnkiart, et al.
Testing, 6, e27526-e27526 (2011)
Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation.
Belay, Eyayu, et al.
Stem Cells, 28, 1760-1771 (2010)
Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
Ficz G. et al.
Nature null
Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.
Kanhere, Aditi, et al.
Molecular Cell, 38, 675-688 (2010)
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