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Key Documents

CBL197F

Sigma-Aldrich

Anti-Cytokeratin 14 Antibody, clone LL002, FITC conjugated

clone LL002, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

FITC conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

LL002, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable (paraffin)

Isotype

IgG3

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KRT14(3861)

Spécificité

Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

FUSION PARTNER: NS1 myeloma cell line

Immunogène

Thyroglobulin conjugated synthetic peptide of extreme carboxy terminus of human keratin 14 (last 15 residues)

Application

Research Category
Cell Structure
Research Sub Category
Cytokeratins
Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.

Optimal working dilutions must be determined by the end user.
This Anti-Cytokeratin 14 Antibody, clone LL002, FITC conjugated is validated for use in IH(P) for the detection of Cytokeratin 14.

Forme physique

The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1 mg/mL bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

Stockage et stabilité

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Aldo Pezzuto et al.
Journal of thoracic disease, 10(7), E570-E576 (2018-09-04)
In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells (CTCs) are better tools
Constitution of fibrin-based niche for in vitro differentiation of adipose-derived mesenchymal stem cells to keratinocytes.
Sivan, U; Jayakumar, K; Krishnan, LK
BioResearch Open Access null

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