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Key Documents

AB9868

Sigma-Aldrich

Anti-Orai1 Antibody, Intracellular

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ORAI1(84876)

Description générale

TRP (transient receptor potential)-related ion channels (TRPCs) have been postulated as candidates for forming both receptor-operated (ROCE) and capacitative Ca2+ entry channels across the plasma membrane. ER transmembrane protein STIM1 senses the depletion of Ca2+ from ER stores. Orai, a regulatory β-subunit of the TRPC ion channel α-subunit, transduces the STIM1 signal into activation of the TRPC. Thus, Orai proteins by interacting with TRPCs act as regulatory subunits that confer STIM1-mediated store depletion sensitivity to these channels and regulate Ca2+ influx through the plasma membrane.

Spécificité

ORai1, intracellular. Detects both the glycosylated and unglycosylated forms of ORai1. By Western blot the antibody reacts with the ~ 50 kDa ORai1 protein. Additional bands between 35 and 50 kDa may be seen if analyzing overexpressed ORai1. These additional bands most likely represent different degrees of glycosylation of ORai1

Immunogène

Synthetic Peptide from the Intracellular domain of Orai1

Application

Immunoblotting: 1:1,000-1:5,000
Immunofluorescence: 1:1,000-1:2,500
Immunoprecipitation: 1 µg
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
This Anti-Orai1 Antibody, Intracellular is validated for use in WB for the detection of Orai1.

Description de la cible

50 Kda

Forme physique

Affinity purified immunoglobulin in PBS with 0.1% NaN3
Format: Purified

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sung Hee Lee et al.
Oncotarget, 7(28), 43239-43255 (2016-06-04)
Emerging evidence indicates that Orai1, a key calcium channel for store-operated Ca2+ entry, is associated with human cancer. However, the underlying mechanism by which Orai1 regulates cancer progression remains unknown. Here we report that intracellular level of Orai1 is increased
Beibei Wu et al.
eLife, 10 (2021-12-16)
CRAC channel regulator 2 A (CRACR2A) is a large Rab GTPase that is expressed abundantly in T cells and acts as a signal transmitter between T cell receptor stimulation and activation of the Ca2+-NFAT and JNK-AP1 pathways. CRACR2A has been
Jimena Laporta et al.
PloS one, 9(10), e110190-e110190 (2014-10-10)
Lactation is characterized by massive transcellular flux of calcium, from the basolateral side of the mammary alveolar epithelium (blood) into the ductal lumen (milk). Regulation of calcium transport during lactation is critical for maternal and neonatal health. The monoamine serotonin
Stefanie L Makowski et al.
Molecular and cellular biology, 35(2), 451-467 (2014-11-12)
The signal peptide peptidase (SPP)-related intramembrane aspartyl proteases are a homologous group of polytopic membrane proteins, some of which function in innate or adaptive immunity by cleaving proteins involved in antigen presentation or intracellular signaling. Signal peptide peptidase-like 3 (SPPL3)
Beibei Wu et al.
Cell reports, 36(8), 109603-109603 (2021-08-26)
Sustained activation of the Ca2+-release-activated Ca2+ (CRAC) channel is pivotal for effector T cell responses. The mechanisms underlying this sustainability remain poorly understood. We find that plasma membrane localization of ORAI1, the pore subunit of CRAC channels, is limited in effector

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