A-004-M
Poly-ʟ-Ornithine
synthetic, liquid, 0.1 mg/mL, suitable for cell culture
Synonyme(s) :
Poly-L-Ornithine Solution (0.01%), A-004-M, Sigma-Aldrich
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About This Item
Code UNSPSC :
12352209
eCl@ss :
32160801
Nomenclature NACRES :
NA.75
Produits recommandés
product name
Poly-L-Ornithine Solution (0.01%),
Taille de l'aiguille
2.0 mm
Niveau de qualité
Stérilité
sterile
Forme
liquid
Poids mol.
Mw 78000 Da
Fabricant/nom de marque
NovaSeptum®
Concentration
0.01 % (w/v)
0.1 mg/mL
Technique(s)
cell attachment: suitable
cell culture | mammalian: suitable
Description générale
Research Category: Stem Cell Research
Research Sub Category: Adhesion (CAMs)
Research Sub Category: Adhesion (CAMs)
Application
Poly-L-Ornithine Solution (0.01%) has been used to coat:
- glass-bottom plates for cell plating in a research study investigating the localization of cMET in MDA-MB-468 cells using confocal microscopy
- culture dishes for adult human neural stem cells (ahNSCs) primary culture
- 6-well plates for induced pluripotent stem cells (iPSCs) culture and neuronal differentiation
Qualité
Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.
Forme physique
Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.
Notes préparatoires
1. Thaw Poly-L-Ornithine solution at room temperature.
2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow the cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.
2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow the cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.
Stockage et stabilité
Store at -20°C for up to 4 months from date of receipt. Avoid multiple freeze thaw cycles to maintain product integrity.
Informations légales
NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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