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Sigma-Aldrich

Phosphatase Inhibitor Cocktail I

liquid, for the inhibition of alkaline phosphatases and serine/threonine protein phosphatases

Synonyme(s) :

Phosphatase inhibitor

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.28

product name

Phosphatase Inhibitor Cocktail Set I, Phosphatase Inhibitor Cocktail Set I contains three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A.

Niveau de qualité

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product is provided as a set of five 1 ml vials. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (Cat. No. 210155; 500 µM), and Microcystin-LR (Cat. No. 475815; 500 nM).
A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product is provided as a set of five 1 ml vials. May be used with cell lysates and tissue extracts, including samples containing detergents. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components:

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
Alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A
Product does not compete with ATP.
Reversible: no

Forme physique

In DMSO.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). Dilute 1:100 just prior to use.

Informations légales

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Point d'éclair (°C)

87 °C - closed cup - (Dimethylsulfoxide)


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Rama K Mishra et al.
Chemical biology & drug design, 94(4), 1813-1823 (2019-07-02)
Mnk kinases (Mnk1 and 2) are downstream effectors of Map kinase pathways and regulate phosphorylation of eukaryotic initiation factor 4E. Engagement of the Mnk pathway is critical in acute myeloid leukemia (AML) leukemogenesis and Mnk inhibitors have potent antileukemic properties
An Phu Tran Nguyen et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(29), 17296-17307 (2020-07-08)
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of late-onset, autosomal-dominant familial Parkinson's disease (PD). LRRK2 functions as both a kinase and GTPase, and PD-linked mutations are known to influence both enzymatic activities. While PD-linked LRRK2
Agnes S Zybura et al.
The Journal of biological chemistry, 295(33), 11845-11865 (2020-07-03)
Nav1.6 is the primary voltage-gated sodium channel isoform expressed in mature axon initial segments and nodes, making it critical for initiation and propagation of neuronal impulses. Thus, Nav1.6 modulation and dysfunction may have profound effects on input-output properties of neurons
Tiffany A McLamarrah et al.
The Journal of cell biology, 219(2) (2019-12-17)
During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6.
Hajime Maeda et al.
Respiratory research, 23(1), 340-340 (2022-12-11)
Premature infants, subjected to supplemental oxygen and mechanical ventilation, may develop bronchopulmonary dysplasia, a chronic lung disease characterized by alveolar dysplasia and impaired vascularization. We and others have shown that hyperoxia causes senescence in cultured lung epithelial cells and fibroblasts.

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