1.15934

Eosin B (bluish) (C.I.45400)

for microscopy Certistain^®

• Synonyme(s) : Eosin B (bluish) (C.I.45400), Dibromodinitrofluorescein sodium
• Formule empirique (notation de Hill): C₂₀H₆Br₂N₂Na₂O₉
• Numéro CAS: 548-24-3
• Poids moléculaire : 624.06
• Numéro MDL: MFCD00005041
• Code UNSPSC : 41116130
• Numéro de classement CE : 208-943-1

Propriétés

• Niveau de qualité: 400
• Forme: solid
• Composition: Dye, ≥85% spectrophotometric assay
• pH: 6.2 (25 °C, 10 g/L in H₂O)
• Solubilité: 300 g/L
• Masse volumique apparente: 680 kg/m³
• εmax: 800-1000 at 517-525 nm in water at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• Clé InChI: GYYTYUGGVBYJHE-UHFFFAOYSA-L

Description

Description générale

Eosin B (C.I. 45400) for microscopy Certistain^®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red. As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Dye content (gravimetrically): ≥ 85 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): 800 - 1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°C): Not applicable