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Key Documents

06-570-AF488

Sigma-Aldrich

Anti-phospho Histone H3 (Ser10) Antibody, Alexa Fluor 488 Conjugate

from rabbit, ALEXA FLUOR 488

Synonyme(s) :

H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

ALEXA FLUOR 488

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human, mouse

Technique(s)

immunocytochemistry: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pSer10)

Informations sur le gène

human ... HIST1H3F(8968)

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogène

Linear peptide corresponding to phospho-Histone H3 (Ser10).

Application

Anti-phospho-H3 (Ser10), Alexa Fluor 488 Conjugate is a purified rabbit polyclonal that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 488 and validated in IC. The unconjugated version of this antibody is highly published and proven in ICC, IP & WB.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Ser10) in NIH/3T3 cells.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cell lysate.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualité

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.

Alexa Fluor is a registered trademark of Life Technologies.

Description de la cible

~17 kDa observed

Forme physique

Protein A purified
Purified rabbit conjugate in buffer containing PBS, 15 mg/ml BSA, and 0.1% Sodium Azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Edward E Large et al.
PLoS genetics, 13(5), e1006769-e1006769 (2017-05-12)
Most biological traits and common diseases have a strong but complex genetic basis, controlled by large numbers of genetic variants with small contributions to a trait or disease risk. The effect-size of most genetic variants is not absolute and is
Baiping Cui et al.
Cell regeneration (London, England), 11(1), 9-9 (2022-04-02)
Myocardial regeneration has been considered a promising option for the treatment of adult myocardial injuries. Previously, a chick early amniotic fluid (ceAF) preparation was shown to contain growth-related factors that promoted embryonic growth and cellular proliferation, though the nature of
Baiping Cui et al.
Frontiers in physiology, 10, 607-607 (2019-06-14)
Adult mammalian heart repair after myocardial damage is highly inefficient due to the post-mitotic nature of cardiomyocytes. Interestingly, in traditional Chinese medicine (TCM), there are reported effective treatments of myocardial infarction (MI) and heart failure in adult humans by oral
Xinlu Gao et al.
International journal of medical sciences, 19(8), 1254-1264 (2022-08-06)
Mammalian cardiomyocytes (CMs) maintain a low capacity for self-renewal in adulthood, therefore the induction of CMs cycle re-entry is an important approach to promote myocardial repair after injury. Recently, photobiomodulation (PBM) has been used to manipulate physiological activities of various
Matheus Henrique Dias et al.
Cancer discovery, 14(7), 1276-1301 (2024-03-27)
Cancer homeostasis depends on a balance between activated oncogenic pathways driving tumorigenesis and engagement of stress response programs that counteract the inherent toxicity of such aberrant signaling. Although inhibition of oncogenic signaling pathways has been explored extensively, there is increasing

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