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100500P

Avanti

E. coli Extract Total

Avanti Research - A Croda Brand

Synonyme(s) :

E. coli Total Lipid Extract

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About This Item

Numéro CAS:
Code UNSPSC :
12352211
Nomenclature NACRES :
NA.25

Forme

powder

Conditionnement

pkg of 1 × 1 g (100500P-1g)
pkg of 1 × 100 mg (100500P-100mg)
pkg of 5 × 100 mg (100500P-500mg)

Fabricant/nom de marque

Avanti Research - A Croda Brand

Type de lipide

lipid extracts

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total has been used in the preparation of unilamellar vesicles in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins and in the reconstitution of proteoliposomes.

Actions biochimiques/physiologiques

The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.

Conditionnement

5 mL Clear Glass Sealed Ampule (100500P-100mg)
5 mL Clear Glass Sealed Ampule (100500P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100500P-1g)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Souvent commandé avec ce produit

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Nji E, et al.
Nature Communications, 9(1), 4253-4253 (2018)
Membrane insertion of small proteins
Protein Secretion, 39-62 (2010)
The extracellular gate shapes the energy profile of an ABC exporter
Hutter CAJ, et al.
Nature Communications, 10(1), 2260-2260 (2019)
Bettina Zens et al.
Methods (San Diego, Calif.), 75, 37-43 (2014-12-03)
Macroautophagy is a major bulk degradation pathway for cytoplasmic material in eukaryotic cells. During macroautophagy, double membrane-bound organelles called autophagosomes are formed in a de novo manner. In the course of their formation autophagosomes capture cytoplasmic material, which is subsequently
Marijke Brams et al.
eLife, 9 (2020-01-29)
Pentameric ligand-gated ion channels (pLGICs) or Cys-loop receptors are involved in fast synaptic signaling in the nervous system. Allosteric modulators bind to sites that are remote from the neurotransmitter binding site, but modify coupling of ligand binding to channel opening.

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