Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

GF83400364

Nickel, foil, not light tested, 100x100mm, thickness 0.002mm, 99.95%

GF25123159

Nickel, foil, 0.5m coil, thickness 0.0125mm, annealed, 99%

GF64926153

Nickel, foil, 10mm disks, thickness 0.05mm, 99.9%

GF67355624

Nickel, foil, 0.5m coil, thickness 0.025mm, 99.9%

GF90435887

Nickel, foil, 0.5m coil, thickness 0.20mm, annealed, 99%

GF54065077

Nickel, foil, 10m coil, thickness 0.05mm, coil width 2mm, annealed, 99%

GF44673183

Nickel, foil, 0.5m coil, thickness 0.075mm, annealed, 99%

GF93488596

Nickel, foil, 10mm disks, thickness 0.03mm, 99.9%

GF16803075

Nickel, foil, 2m coil, thickness 0.125mm, annealed, 99.98%

GF19347521

Nickel, wire reel, 100m, diameter 0.5mm, as drawn, 99%

GF20895903

Nickel, microfoil, disks, 25mm, thinness 1.0μm, specific density 895.8μg/cm², permanent mylar 3.5μm support, 99.99+%

GF13212807

Nickel, pellets, 50g, max. size 5mm, 99.99+%

GF73011833

Nickel, foil, 50x50mm, thickness 1.0mm, annealed, 99.98%

GF74649695

Nickel, foil, 2m coil, thickness 0.3mm, annealed, 99%

GF51323093

Nickel, tube, 500mm, outside diameter 3.6mm, inside diameter 3.5mm, wall thickness 0.05mm, hard, 99.5%

GF07087439

Nickel, tube, 500mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%

GF22004334

Nickel, foil, 150x300mm, thickness 0.5mm, annealed, 99.98%

GF70704738

Nickel, wire reel, 10m, diameter 0.125mm, hard, 99.98%

GF28374767

Nickel, foil, not light tested, 50x50mm, thickness 0.007mm, 99.95%

GF66058612

Nickel, foil, 10mm disks, thickness 0.025mm, 99.9%

GF89699315

Nickel, tube, 500mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, hard, 99.5%

GF00192327

Nickel, insulated wire, 20m, conductor diameter 0.025mm, insulation thickness 0.0035mm, polyimide insulation

GF15349990

Nickel, tube, 500mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, hard, 99.5%

GF87059021

Nickel, foil, 25x25mm, thickness 0.125mm, as rolled, 99.999%