GenElute™-E DNA and RNA Purification Kits

Traditional silica-based spin prep kits require multiple, tedious wash and spin steps with lots of hands-on tube manipulation and little downtime. GenElute^™-E Single Spin DNA and RNA Purification Kits use a negative chromatography method based on size exclusion for the purification of genomic DNA (gDNA) from mammalian cells, blood, tissue, and plant tissue samples as well as for DNA and RNA cleanup procedures.

• GenElute™-E DNA and RNA Extraction Single Spin Technology
• PCR Optimization with GenElute™-E DNA and RNA Purification Kits Webinar
• GenElute™-E Viral RNA/DNA Kits for SARS-CoV-2 Viral RNA Extraction and Purification

GenElute™-E DNA and RNA Purification Single Spin Technology

In this size exclusion chromatography method, molecules in the sample are separated by size. Smaller molecules such as proteins, lipids, and ionic components become trapped in the pores of the stationary phase of the column. Larger molecules such as DNA and RNA flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute^™-E purification kits enable DNA and RNA purification in a single spin without the need for multiple binding and wash steps.

Download Infographic: Discover how GenElute^™-E Single Spin technology enables nucleic acid purification in a single step!

Kits are optimized to provide high yield, high concentration, and high purity DNA and RNA for downstream applications such as PCR, qPCR, multiplex PCR, RT-PCR, and next generation sequencing (NGS).

 

PCR Optimization with GenElute™-E DNA and RNA Purification Kits Webinar

PCR is a beneficial laboratory tool; however, its efficiency is only as robust as the reaction parts, including the nucleic acid template. This webinar demonstrates the benefits of a new negative chromatography method of nucleic acid isolation using GenElute™-E Single Spin technology.

GenElute™-E Viral RNA/DNA Kits for SARS-CoV-2 Viral RNA Extraction and Purification

Rapid and reliable viral RNA extraction and purification technology is essential for disease research, especially for timely results involving SARS-CoV-2 (COVID-19). GenElute™-E Viral RNA/DNA kits provide a streamlined workflow to isolate RNA and DNA from nasopharyngeal swabs, genital swabs, and stool samples. Compatible with dry swabs and a variety of transport media, samples can be processed with a single spin, and purified RNA and DNA can be analyzed in downstream applications like PCR, qPCR, and sequencing. Additionally, GenElute™-E Viral RNA/DNA Kits were evaluated using SARS-CoV-2 RNA swab samples (Figure 1) and stool samples (Figure 2) in qPCR applications.

For Research Use Only. Not For Use In Diagnostic Procedures. Product not available for sale in Europe.

SARS-CoV-2 analysis: Swab Sample qPCR Cq Value Differentiation Between Copy Numbers

Figure 1. Successful participation in an interlaboratory test by INSTAND e.V. Scatter plot analysis reveals a significant (p < 0.0001) differentiation between reference sample 1 (1x107 copies/mL) and sample 2 (1x106 copies mL) in a combination of the GenElute™-E Viral RNA/DNA Kit with either the (A) RIDA^® GENE SARS-CoV-2 (R-Biopharm) or the (B) SARS-CoV-2 PCR Kit (Anchor Diagnostics) for E gene. A dilution factor of 7.3 and 7.8 was shown for R-Biopharm (A) and Anchor Diagnostics (B) assays, respectively, which is within the calculated range across 234 labs that participated in the interlaboratory test. A 95% Confidence Interval (CI) is shown for each of the reference samples.

Viral Detection from Stool Samples: Performance in qPCR

Table 1. Clinical validation for human norovirus extraction was investigated on a total of 83 patient samples. The GenElute™-E Viral RNA/DNA Kit was used in combination with the RIDA^®GENE Viral Stool Panel I (R-Biopharm), which is a multiplex real-time RT-PCR for the direct, qualitative detection and differentiation of Norovirus, Rotavirus, Adenovirus, and Astrovirus in human stool samples.

For additional information and related DNA and RNA extraction protocols, please visit our DNA and RNA purification application page or discover our reliable reagents for your gDNA purification and RNA purification workflow needs.

GenElute™-E Viral RNA/DNA kits provide a streamlined workflow to isolate RNA and DNA from nasopharyngeal swabs, genital swabs, and stool samples. Compatible with dry swabs and a variety of transport media, samples can be processed with a single spin and purified RNA and DNA can be analyzed in downstream applications like PCR, qPCR, and sequencing.

For Research Use Only. Not For Use In Diagnostic Procedures. Products EC810, EC848, EC888, and EC896 not available for sale in Europe.