Iscove's Modified Dulbecco's Media (IMDM) Formulation
| Component | I3390 [1x] g/L | I7633 g/L |
|---|---|---|
| Inorganic Salts | ||
| Calcium Chloride | 0.1653 | 0.1653 |
| Magnesium Sulfate (anhydrous) | 0.09767 | 0.9767 |
| Potassium Chloride | 0.33 | 0.33 |
| Potassium Nitrate | 0.000076 | 0.000076 |
| Sodium Bicarbonate | 3.024 | — |
| Sodium Chloride | 4.505 | 4.505 |
| Sodium Phosphate Monobasic (anhydrous) | 0.109 | 0.109 |
| Sodium Selenite | 0.000017 | 0.000017 |
| Amino Acids | ||
| L-Alanine | 0.025 | 0.025 |
| L-Arginine • HCl | 0.084 | 0.084 |
| L-Asparagine • H2O | 0.0284 | 0.0284 |
| L-Aspartic Acid | 0.03 | 0.03 |
| L-Cystine • 2HCl | 0.09124 | 0.09124 |
| L-Glutamic Acid | 0.075 | 0.075 |
| L-Glutamine | — | 0.584 |
| Glycine | 0.03 | 0.03 |
| L-Histidine • HCl • H2O | 0.042 | 0.042 |
| L-Isoleucine | 0.105 | 0.105 |
| L-Leucine | 0.105 | 0.105 |
| L-Lysine • HCl | 0.146 | 0.146 |
| L-Methionine | 0.03 | 0.03 |
| L-Phenylalanine | 0.066 | 0.066 |
| L-Proline | 0.04 | 0.04 |
| L-Serine | 0.042 | 0.042 |
| L-Threonine | 0.095 | 0.095 |
| L-Tryptophan | 0.016 | 0.016 |
| L-Tyrosine • 2Na • 2H2O | 0.10379 | 0.10379 |
| L-Valine | 0.094 | 0.094 |
| Vitamins | ||
| D-Biotin | 0.000013 | 0.000013 |
| Choline Chloride | 0.004 | 0.004 |
| Folic Acid | 0.004 | 0.004 |
| myo-Inositol | 0.0072 | 0.0072 |
| Niacinamide | 0.004 | 0.004 |
| D-Pantothenic Acid (hemicalcium) | 0.004 | 0.004 |
| Pyridoxal • HCl | 0.004 | 0.004 |
| Riboflavin | 0.0004 | 0.0004 |
| Thiamine • HCl | 0.004 | 0.004 |
| Vitamin B12 | 0.000013 | 0.000013 |
| Other | ||
| D-Glucose | 4.5 | 4.5 |
| HEPES | 5.958 | 5.958 |
| Phenol Red • Na | 0.016 | 0.016 |
| Pyruvic Acid • Na | 0.11 | 0.11 |
| Add | ||
| Sodium Bicarbonate | — | 3.024 |
| L-Glutamine | 0.584 | — |
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