HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
Materials
analytical column
mobile phase component
Product No.
Description
Pricing
Sodium chloride
for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)standard
Product No.
Description
Pricing
CONDITIONS
column
TSKgel Bio Assist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)
column
Competitive S-type column, 5 cm x 5 mm I.D.
mobile phase
[A] 20 mM sodium phophate buffer, pH 7.0; [B] 20 mM sodium phophate buffer, 0.5 M NaCl, pH 7.0
gradient
0 to 100% B in 20 min (0 to 100% B in 30 min for competitive column)
flow rate
0.8 mL/min (1.0 mL/min for competitive column)
column temp.
25 °C
detector
UV, 280 nm
sample
crude bromelain, 0.01 μg/mL
Description
Other Notes
Data provided by Tosoh Bioscience LLC.
Legal Information
BioAssist is a registered trademark of Tosoh Corporation
TSKgel is a registered trademark of Tosoh Corporation