- Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.
Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.
Science (New York, N.Y.) (1966-08-12)
N Zwaig, E C Lin
PMID5328677
ABSTRACT
Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which synthesized constitutively such an altered enzyme, even though the usual degree of catabolite repression still operated.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Glycerokinase from Bacillus stearothermophilus, buffered aqueous solution, ≥75 units/mg protein (biuret)
Sigma-Aldrich
Glycerokinase from Escherichia coli, 300-600 units/mL, ammonium sulfate suspension