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  • Oncogenic Protein Kinase D3 Regulating Networks in Invasive Breast Cancer.

Oncogenic Protein Kinase D3 Regulating Networks in Invasive Breast Cancer.

International journal of biological sciences (2017-06-29)
Yan Liu, Jian Li, Jun Zhang, Zhenghong Yu, Shiyi Yu, Lele Wu, Yuzhi Wang, Xue Gong, Chenxi Wu, Xiuxiu Cai, Lin Mo, Mingya Wang, Jun Gu, Liming Chen
ABSTRACT

Protein Kinase D3 (PRKD3) functions as an important oncogenic driver in invasive breast cancer, which is the leading cause of women mortality. However, PRKD3 regulating network is largely unknown. In this study, we systematically explored PRKD3 regulating networks via investigating phosphoproteome, interactome and transcriptome to uncover the molecular mechanism of PRKD3 in invasive breast cancer. Using iTRAQ, 270 proteins were identified as PRKD3 regulated phosphoproteins from 4619 phosphosites matching 3666 phosphopeptides from 2016 phosphoproteins with p-value <0.005. Transcriptome analysis using affymetrix microarray identified 45 PRKD3 regulated genes, in which 20 genes were upregulated and 25 genes were downregulated with p-value <0.005 upon silencing