Skip to Content
Merck
  • Hydrophobic-hydrophilic monolithic dual-phase layer for two-dimensional thin-layer chromatography coupled with surface-enhanced Raman spectroscopy detection.

Hydrophobic-hydrophilic monolithic dual-phase layer for two-dimensional thin-layer chromatography coupled with surface-enhanced Raman spectroscopy detection.

Journal of separation science (2015-05-13)
Binxing Zheng, Yanhua Liu, Dan Li, Yifeng Chai, Feng Lu, Jiyang Xu
ABSTRACT

Hydrophobic-hydrophilic monolithic dual-phase plates have been prepared by a two-step polymerization method for two-dimensional thin-layer chromatography of low-molecular-weight compounds, namely, several dyes. The thin 200 μm poly(glycidyl methacrylate-co-ethylene dimethacrylate) layers attached to microscope glass plates were prepared using a UV-initiated polymerization method within a simple glass mold. After cutting and cleaning the specific area of the layer, the reassembled mold was filled with a polymerization mixture of butyl methacrylate and ethylene dimethacrylate and subsequently irradiated with UV light. During the second polymerization process, the former layer was protected from the UV light with a UV mask. After extracting the porogens and hydrolyzing the poly(glycidyl methacrylate-co-ethylene dimethacrylate) area, these two-dimensional layers were used to separate a mixture of dyes with great difference in their polarity using reversed-phase chromatography mode within the hydrophobic layer and then hydrophilic interaction chromatography mode along the hydrophilic area. In the latter dimension only the specific spot was developed further. Detection of the separated dyes could be achieved with surface-enhanced Raman spectroscopy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sulfuric acid, 99.999%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hydrochloric acid solution, ~6 M in H2O, for amino acid analysis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 0.85%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methyl Red, ACS reagent, crystalline
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trisodium citrate dihydrate, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Supelco
Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, tablet
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride solution, 5 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide, AR, pellets, ≥98.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium hydroxide, ultra dry, powder or crystals, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hydrochloric acid solution, 32 wt. % in H2O, FCC
SKU
Pack Size
Availability
Price
Quantity