Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Cell proliferation (2014-04-18)

G He, B Guo, H Wang, C Liang, L Ye, Y Lin, X Cai

ABSTRACT

To improve efficacy of current titanium and its alloys, in bioactivity and speed of osseointegration, of orthopaedic implants. A novel triple-layered functional graded coating, consisting of a porous hydroxyapatite (HA) outermost layer, fluoro-HA (FHA) intermediate layer and titanium oxide (TiO2 ) innermost layer, was created on a titanium substrate by a multistep sol-gel method. X-ray diffraction analysis showed TiO2 anatase and apatite crystallization in the coating. Morphological analysis performed by scanning electron microscopy showed excellent bonding between coating and substrate, with a thickness of ~2 μm. Scratch testing found favourable adhesion strength of the composite coating. In addition, optical microscope images suggested good biocompatibility. Considering thet in vitro cell response, osteoblasts on the coating exhibited higher cell proliferation and ALP activity compared to pure titanium and HA coating, and demonstrated excellent coating bioactivity. Current results indicated that the novel TiO2 /FHA/HA coating has promising clinical applications in orthopaedic and dental implantation.

MATERIALS

Product Number

Brand

Product Description

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

H0252

Sigma-Aldrich

Hydroxyapatite, Type I, buffered aqueous suspension

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

348848

Sigma-Aldrich

Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

204730

Sigma-Aldrich

Titanium(IV) oxide, rutile, 99.995% trace metals basis

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

635065

Sigma-Aldrich

Titanium(IV) oxide, rutile, <110>, single crystal substrate, ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

700339

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <100 nm particle size, dispersion, 48-52 wt. % in xylene, 99.9% trace metals basis

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

700355

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticle, <250 nm particle size (DLS), paste, 53-57 wt. % in diethylene glycol monobutyl ether/ethylene glycol, 99.9% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

774529

Sigma-Aldrich

Titanium(IV) oxide, nanowires, diam. × L ~10 nm × 10 μm

677469

Sigma-Aldrich

Titanium(IV) oxide, contains 1% Mn as dopant, nanopowder, <100 nm particle size (BET), ≥97%

IRMM531A

Titanium, IRMM^®, certified reference material, 0.1 mm foil

268526