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  • Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

Journal of medicinal food (2012-10-17)
Fernanda Nedel, Karine Begnini, Pedro Henrique de Azambuja Carvalho, Rafael Guerra Lund, Fátima T A Beira, Francisco Augusto B Del Pino
ABSTRACT

This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sulforhodamine B, Dye content 75 %
Sigma-Aldrich
Sulforhodamine B, acid form, laser grade, Dye content 95 %
Supelco
Hexane, analytical standard
Sigma-Aldrich
Hexane-1-13C, 99 atom % 13C
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Sulforhodamine B sodium salt, Technical grade
Sigma-Aldrich
Sulforhodamine B sodium salt, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Hexane, suitable for HPLC, ≥95%
Sigma-Aldrich
Hexane, anhydrous, 95%
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Hexane, suitable for HPLC, ≥97.0% (GC)
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Hexane, ReagentPlus®, ≥99%
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Hexane, Laboratory Reagent, ≥95%
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Hexane, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
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Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%