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Methylation-specific PCR.

Methods in molecular biology (Clifton, N.J.) (2011-09-14)
Ja-Lok Ku, You-Kyung Jeon, Jae-Gahb Park
ABSTRACT

DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium bisulfite solution, purum, ~40%
Sigma-Aldrich
Sodium bisulfite, ACS reagent
Sigma-Aldrich
Sodium bisulfite, anhydrous, free-flowing, Redi-Dri, ACS reagent